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Article

Quantification and genotyping of hepatitis B virus in a single reaction by real-time PCR and melting curve analysis.

Journal of hepatology - 1 Oct 2004

Yeh Shiou-Hwei, Tsai Ching-Yi, Kao Jia-Horng, Liu Chun-Jen, Kuo Ti-Jung, Lin Ming-Wei, Huang Wen-Ling, Lu Shu-Fen, Jih Jane, Chen Ding-Shinn, Chen Pei-Jer

Abstract excerpt

BACKGROUND/AIMS: Both viral titer and genotype of hepatitis B virus (HBV) play critical roles in determining clinical outcome and response to antiviral treatment in hepatitis B patients. In this study, a method was developed to determine both parameters in a single-tube reaction. METHODS: The method contains two consecutive steps, the first step used real-time PCR for quantification and second step used melting...

Topics

Computer Systems
DNA Primers
Genotype
Hepatitis B
Hepatitis B virus
Humans
Molecular Probes
Polymerase Chain Reaction
Viral Load
Virology

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 15464248
DOI: 10.1016/j.jhep.2004.06.031

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