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Article

Universal molecular beacon-based tracer system for real-time polymerase chain reaction.

Analytical chemistry - 15 Nov 2006

Li Xiaomin, Huang Yong, Guan Yuan, Zhao Meiping, Li Yuanzong

Abstract excerpt

DNA diagnostic has been moving from expensive, low-throughput, multistep methods to inexpensive, higher throughput, closed-tube, and automated methods. Fluorescence is the favored signaling technology for such assays. In this method, we describe a universal molecular beacon (U-MB) as the fluorescent tracer in the real-time PCR technique. A 5'-universal template primer (5'-UT primer) has been designed with a tail...

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PMID: 17105184
DOI: 10.1021/ac061518+

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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