Determination of mutated genes in the presence of wild-type DNA by using molecular beacons as probe.

doi:10.1016/j.saa.2016.11.050

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Article

Determination of mutated genes in the presence of wild-type DNA by using molecular beacons as probe.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy - 5 Mar 2017

Zhang Yonghua, Ai Junjie, Gu Qiaorong, Gao Qiang, Qi Honglan, Zhang Chengxiao

Abstract excerpt

Low-abundance mutations in the presence of wild-type DNA can be determined using molecular beacon (MB) as probe. A MB is generally used as DNA probe because it can distinguish single-base mismatched target DNA from fully matched target DNA. However, the probe can not determine low-abundance mutations in the presence of wild-type DNA. In this study, this limitation is addressed by enhancing the stability of...

Topics

Circular Dichroism
DNA
Genes
Molecular Probes
Mutation
Nucleic Acid Denaturation
Polymerase Chain Reaction
Polymorphism, Single Nucleotide
Spectrometry, Fluorescence
Temperature

Source

Open in PubMed

Identifiers

PMID: 27960142
DOI: 10.1016/j.saa.2016.11.050

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