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Article

Protocol for the use of self-reporting duplex mutation primers to detect PCR products in the diagnosis of HBV.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2012

Xia Qian-Feng

Abstract excerpt

Quantitative measurements of serum hepatitis B virus (HBV) DNA are useful for tailoring of treatment schedules and the monitoring of HBV replication during therapy. We developed a novel fluorescence-based quantitative real-time PCR for quantitating HBV DNA based on the duplex mutation primers principle, in which signal is generated by melting a duplex mutation primer during renaturation. The duplex mutation...

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PMID: 22782823
DOI: 10.1007/978-1-61779-937-2_16

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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