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Article

Detection of YMDD mutants using universal template real-time PCR.

World journal of gastroenterology - 28 Feb 2006

Wang Rong-Sheng, Zhang Hua, Zhu Yu-Fen, Han Bei, Yang Zhi-Jun

Abstract excerpt

AIM: To establish a rapid and accurate method for the detection of lamivudine-resistant mutations in hepatitis B virus and monitor of lamivudine resistance during lamivudine treatment in patients with chronic hepatitis B virus infection. METHODS: We established a real-time PCR method using a universal template and TaqMan probe to detect YMDD mutants. Variants of YVDD and YIDD were tested by individual reactions...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 16534892
DOI: 10.3748/wjg.v12.i8.1308
PMCID: PMC4124450

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