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Article

A novel one cycle allele specific primer extension--molecular beacon displacement method for DNA point mutation detection with improved specificity.

Analytica chimica acta - 12 Feb 2007

Li Xiaomin, Huang Yong, Guan Yuan, Zhao Meiping, Li Yuanzong

Abstract excerpt

We report here a new method for the real-time detection of DNA point mutations with molecular beacon as the fluorescence tracer and 3' (exo-) Bst DNA polymerase large fragment as the polymerase. The method is based on the mechanism of allele specific primer extension-strand displacement (ASPE-SD). To improve the specificity of the method only one cycle of the allele specific polymerase chain reaction (PCR) was...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 17386579
DOI: 10.1016/j.aca.2006.11.016

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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