Article
Single-base extension and ELISA-based approach for single-nucleotide polymorphisms genotyping.
Applied biochemistry and biotechnology - 1 Mar 2011
Liu Guibo, Cheng Yongxia, Zhao Wei, Jin Zaishun, Shan Hongbo, Xu Gaolian
Abstract excerpt
Single-nucleotide polymorphisms (SNPs) emerge as a fundamental tool in personalized medicine due to their association with drug responses or disease predisposition. Single-base extension (SBE) is a common method for characterizing known SNPs, but involves complicated procedures or requires costly analytical instruments. Here, we describe a novel SNP genotyping based on SBE and enzyme-linked immunosorbent assay...
Topics
- Enzyme-Linked Immunosorbent Assay
- Genotype
- Humans
- Polymerase Chain Reaction
- Polymorphism, Single Nucleotide
