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Article

A star-shaped DNA probe based on strand displacement for universal and multiplexed fluorometric detection of genetic variations.

Mikrochimica acta - 13 Aug 2018

Liu Na, Xu Kai, Liu Liquan, Chen Xin, Zou Yufan, Xiao Xianjin

Abstract excerpt

A star-shaped fluorescent DNA probe (S-probe) is described that can recognize target DNA and discriminate it from interfering DNA via strand displacement including branch migration and toehold exchange. The artificially designed S-probe does not harm the strand displacement while it allows the fluorescently labelled strand and the quencher-labelled strand to be shared among different S-probes targeting different...

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Blood Specimen Collection
DNA
DNA Probes
Fluorescent Dyes
Fluorometry

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Genetic Variation

Limit of Detection

Ovarian Neoplasms

Source

Identifiers

PMID: 30105500
DOI: 10.1007/s00604-018-2941-0

Genes

EGFR
KRAS

Variants

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