Article
Evidence for processivity and two-step binding of the RNA substrate from studies of J1/2 mutants of the Tetrahymena ribozyme.
Biochemistry - 11 Feb 1992
Herschlag D
Abstract excerpt
J1/2 of the Tetrahymena ribozyme, a sequence of three A residues, connects the RNA-binding site to the catalytic core. Addition or deletion of bases from J1/2 improves turnover and substrate specificity in the site-specific endonuclease reaction catalyzed by this ribozyme: G2CCCUCUA5 (S) + G in-e...
Topics
- Animals
- Base Sequence
- Binding Sites
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Oligonucleotides
- RNA Processing, Post-Transcriptional
- RNA, Catalytic
- Structure-Activity Relationship
- Substrate Specificity
- Tetrahymena