Article
Thermostable red and green light-producing firefly luciferase mutants for bioluminescent reporter applications.
Analytical biochemistry - 15 Feb 2007
Branchini Bruce R, Ablamsky Danielle M, Murtiashaw Martha H, Uzasci Lerna, Fraga Hugo, Southworth Tara L
Abstract excerpt
Light emission from the North American firefly Photinus pyralis, which emits yellow-green (557-nm) light, is widely believed to be the most efficient bioluminescence system known, making this luciferase an excellent tool for monitoring gene expression. We present studies on the production of a set of thermostable red- and green-emitting luciferase mutants with bioluminescent properties suitable for dual-color...
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