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Article

Discovery of Red-Shifting Mutations in Firefly Luciferase Using High-Throughput Biochemistry.

Biochemistry - 19 Mar 2024

Colee Clair M, Oberlag Nicole M, Simon Marcell, Chapman Owen S, Flanagan Lyndsey C, Reid-McLaughlin Edison S, Gewing-Mullins Jordan A, Maiche Synaida, Patel Devi F, Cavalcanti Andre R O, Leconte Aaron M

Abstract excerpt

Photinus pyralis luciferase (FLuc) has proven a valuable tool for bioluminescence imaging, but much of the light emitted from the native enzyme is absorbed by endogenous biomolecules. Thus, luciferases displaying red-shifted emission enable higher resolution during deep-tissue imaging. A robust model of how protein structure determines emission color would greatly aid the engineering of red-shifted mutants, but...

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Identifiers

PMID: 38437583
DOI: 10.1021/acs.biochem.3c00708
PMCID: PMC10956436

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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