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Article

Delicate balance of electrostatic interactions and disulfide bridges in thermostability of firefly luciferase.

International journal of biological macromolecules - 1 Dec 2012

Karimzadeh Somayeh, Moradi Maryam, Hosseinkhani Saman

Abstract excerpt

The wild type Photinus pyralis luciferase does not have any disulfide bridge. Disulfide bridges are determinant in inherent stability of protein at moderate temperatures. Meanwhile, arginin is responsible for thermostability at higher temperatures. In this study, by concomitant introduction of disulfide bridge and a surface arginin in a mutant (A296C-A326C/I232R), the contribution of disulfide bridge introduction...

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PMID: 22750581
DOI: 10.1016/j.ijbiomac.2012.06.028

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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