Mutation of conserved residues K329 and R330 on the surface of firefly luciferase: Effect on proteolytic degradation.

Abstract excerpt

Firefly luciferase is highly susceptible to proteolytic digestion that reduces its half-life and leads to loss in sensitivity. Due to the protease contamination in most in vitro and in vivo environments, it has interest to generate some mutations that may lead to improved susceptibility to digestion. Some important conserved residues (including K206, R213, R218, K329, R330 and R337) on accessible and flexible...

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