Article
Mutagenesis of solvent-exposed amino acids in Photinus pyralis luciferase improves thermostability and pH-tolerance.
The Biochemical journal - 15 Jul 2006
Law G H Erica, Gandelman Olga A, Tisi Laurence C, Lowe Christopher R, Murray James A H
Abstract excerpt
Firefly luciferase catalyses a two-step reaction, using ATP-Mg2+, firefly luciferin and molecular oxygen as substrates, leading to the efficient emission of yellow-green light. We report the identification of novel luciferase mutants which combine improved pH-tolerance and thermostability and that retain the specific activity of the wild-type enzyme. These were identified by the mutagenesis of solvent-exposed...
Topics
- Animals
- Fireflies
- Hot Temperature
- Hydrogen-Ion Concentration
- Kinetics
- Luciferases
- Luminescence
- Models, Chemical
- Models, Molecular
- Mutagenesis
- Mutation