Article
Improved thermostability of the North American firefly luciferase: saturation mutagenesis at position 354.
The Biochemical journal - 15 Oct 1996
White P J, Squirrell D J, Arnaud P, Lowe C R, Murray J A
Abstract excerpt
We have used random chemical mutagenesis and a simple genetic screen to generate and isolate a thermostable mutant of luciferase from the North American firefly (Photinus pyralis). A single G-to-A transition mutation, resulting in the substitution of a glutamate for a lysine residue at position 3...
Topics
- Animals
- Coleoptera
- Glutamic Acid
- Luciferases
- Lysine
- Mutagenesis, Site-Directed
- Mutation
- Recombinant Proteins
- Temperature