Article
Mutation of a protease-sensitive region in firefly luciferase alters light emission properties.
The Journal of biological chemistry - 25 Jul 1997
Thompson J F, Geoghegan K F, Lloyd D B, Lanzetti A J, Magyar R A, Anderson S M, Branchini B R
Abstract excerpt
Luciferase (EC 1.13.12.7) from the North American firefly, Photinus pyralis, is widely used as a reporter enzyme in cell biology. One of its distinctive properties is a pronounced susceptibility to proteolytic degradation that causes luciferase to have a very short intracellular half-life. To def...
Topics
- Amino Acid Sequence
- Animals
- Coleoptera
- Electrophoresis, Polyacrylamide Gel
- Hydrogen-Ion Concentration
- Kinetics
- Luciferases
- Luminescent Measurements
- Molecular Sequence Data
- Mutation
- Sequence Homology, Amino Acid