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Article

Design and characterization of novel trypsin-resistant firefly luciferases by site-directed mutagenesis.

Protein engineering, design & selection : PEDS - 1 Nov 2009

Riahi-Madvar Ali, Hosseinkhani Saman

Abstract excerpt

Firefly luciferase (EC.1.13.12.7) from Photinus pyralis is a single polypeptide chain (62 kDa), responsible for emission of yellow-green (557 nm) light, known to be most efficient bioluminescence system that make it an excellent tool for reporter in nano-system biology. However, it is very sensitive to proteolytic degradation, which reduces its intracellular half-life, leads to loss in sensitivity and precision...

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Identifiers

PMID: 19661129
DOI: 10.1093/protein/gzp047

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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