Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A set of multicolored Photinus pyralis luciferase mutants for in vivo bioluminescence applications.

Protein engineering, design & selection : PEDS - 1 Dec 2005

Shapiro Elyse, Lu Connie, Baneyx François

Abstract excerpt

Error-prone PCR was used to isolate Photinus pyralis luciferase mutants producing bright light in the red-orange region of the spectrum. All mutations were clustered in the beta5-alpha10-beta6 region of N-terminal subdomain B and appear to affect bioluminescence color by modulating the position of the Ser314-Leu319 mobile loop with respect to the putative active site. Two red variants (Q283R and S284G) and one...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Enzyme Stability
Share to Escherichia coli
Share to Luciferases

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Luminescent Measurements

Share to Mutagenesis, Site-Directed

Share to Protein Folding

Share to Protein Isoforms

Share to Recombinant Proteins

Source

Identifiers

PMID: 16243898
DOI: 10.1093/protein/gzi066

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A set of multicolored Photinus pyralis luciferase mutants for in vivo bioluminescence applications.PMID 16243898Read ↗

Thermostable red and green light-producing firefly luciferase mutants for bioluminescent reporter applications.Semantic proximity Read ↗

Mutagenesis of solvent-exposed amino acids in Photinus pyralis luciferase improves thermostability and pH-tolerance.Semantic proximity Read ↗

Design and characterization of novel trypsin-resistant firefly luciferases by site-directed mutagenesis.Semantic proximity Read ↗

Discovery of Red-Shifting Mutations in Firefly Luciferase Using High-Throughput Biochemistry.Semantic proximity Read ↗

Strategy of mutual compensation of green and red mutants of firefly luciferase identifies a mutation of the highly conservative residue E457 with a strong red shift of bioluminescence.Semantic proximity Read ↗

Mutation of a protease-sensitive region in firefly luciferase alters light emission properties.Semantic proximity Read ↗

Increase in bioluminescence intensity of firefly luciferase using genetic modification.Semantic proximity Read ↗

The effective role of positive charge saturation in bioluminescence color and thermostability of firefly luciferase.Semantic proximity Read ↗

Improved thermostability of the North American firefly luciferase: saturation mutagenesis at position 354.Semantic proximity Read ↗

Expanding the <i>Bacteroides</i> synthetic biology toolkit to develop an <i>in vivo</i> intestinal malabsorption biosensorSemantic proximity Read ↗

Advanced Bioluminescence Reporter with Engineered Gaussia Luciferase via Sequence-Guided Mutagenesis.Semantic proximity Read ↗

Proteorhodopsin in living color: diversity of spectral properties within living bacterial cells.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.