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Article

Triple substitution G216N/A217L/S398M leads to the active and thermostable Luciola mingrelica firefly luciferase.

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology - 1 Jun 2011

Koksharov Mikhail I, Ugarova Natalia N

Abstract excerpt

Insufficient thermal stability of firefly luciferases often limits their application in a wide range of fields. The substitution A217L is known to greatly increase thermal stability of many firefly luciferases. However, for Hotaria parvula firefly luciferase, that shares 98% degree of homology with Luciola mingrelica luciferase, the A217L mutation is known to dramatically decrease catalytic activity. We analyzed...

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Identifiers

PMID: 21321773
DOI: 10.1039/c0pp00318b

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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