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Article

Increase in bioluminescence intensity of firefly luciferase using genetic modification.

Analytical biochemistry - 15 Jul 2007

Fujii Hiroya, Noda Kenichi, Asami Yasuo, Kuroda Akio, Sakata Minoru, Tokida Akihiko

Abstract excerpt

Firefly luciferase is widely used for enzymatic measurement of ATP, and its gene is used as a reporter for gene expression experiments. From our mutant library, we selected novel mutations in Photinus pyralis luciferase with higher luminescence intensity. These included mutations at Ile423, Asp436, and Leu530. Luciferase is structurally composed of a large N-terminal active site domain (residues 1-436), a...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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PMID: 17540326
DOI: 10.1016/j.ab.2007.04.018

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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