Article
Mutational analysis of the galactose binding ability of recombinant ricin B chain.
The Journal of biological chemistry - 15 Oct 1991
Wales R, Richardson P T, Roberts L M, Woodland H R, Lord J M
Abstract excerpt
Ricin B chain (RTB) is an N-glycosylated galactose-specific lectin which folds into two globular domains. Each domain binds one galactoside. The x-ray crystallographic structure has shown that the two binding sites are structurally similar and contain key binding residues which hydrogen bond to the sugar, and a conserved tripeptide, Asp-Val-Arg. We have used oligonucleotide site-directed mutagenesis to change...
Topics
- Animals
- Asialoglycoproteins
- Electrophoresis, Polyacrylamide Gel
- Fetuins
- Galactose
- Gene Expression
- Glycosylation
- Lactose
- Lectins
- Mutagenesis, Site-Directed
- Mutation