Article
Ricin B chain fragments expressed in Escherichia coli are able to bind free galactose in contrast to the full length polypeptide.
Glycoconjugate journal - 1 Aug 1994
Wales R, Gorham H C, Hussain K, Roberts L M, Lord J M
Abstract excerpt
Deleted forms of ricin B chain (RTB) containing only one of the two galactose binding sites were produced in E. coli and targeted to the periplasm by fusion to the ompA or ompF signal sequences. The proteins were then isolated from the periplasm and their sugar binding properties assessed. Previous studies investigating the properties of such proteins produced in Xenopus laevis oocytes suggested that deleted...
Topics
- Base Sequence
- Cloning, Molecular
- Escherichia coli
- Galactose
- Gene Deletion
- Molecular Sequence Data
- Mutation
- Peptides
- Protein Binding
- Recombinant Proteins
- Ricin