Article
Role of conserved and nonconserved residues in the Ca(2+)-dependent carbohydrate-recognition domain of a rat mannose-binding protein. Analysis by random cassette mutagenesis.
The Journal of biological chemistry - 25 May 1992
Quesenberry M S, Drickamer K
Abstract excerpt
The carbohydrate-recognition domain of rat serum mannose-binding protein A has been subjected to random cassette mutagenesis. Mutant domains, expressed in bacteria, were initially screened for binding to invertase-coated nitrocellulose and then analyzed further for Ca2+ affinity, saccharide bindi...
Topics
- Amino Acids
- Animals
- Calcium
- Carbohydrate Metabolism
- Carrier Proteins
- Cations, Divalent
- Cross-Linking Reagents
- DNA
- Electrophoresis, Polyacrylamide Gel
- Mannose
- Mannose-Binding Lectin
- Mutagenesis