Article
Mutational and structural analysis of the lectin activity in binding domain 2 of ricin B chain.
Protein engineering - 1 Oct 1994
Lehar S M, Pedersen J T, Kamath R S, Swimmer C, Goldmacher V S, Lambert J M, Blättler W A, Guild B C
Abstract excerpt
The study of the lectin binding sites of ricin B chain and of other homologous members of the small gene family that make up ricin-like molecules has revealed a number of key contact residues involved in sugar binding. In particular, on the basis of data generated by the X-ray crystallographic st...
Topics
- Amino Acid Sequence
- Binding Sites
- Coliphages
- Genes, Plant
- Models, Molecular
- Molecular Sequence Data
- Molecular Structure
- Mutagenesis, Site-Directed
- Mutation
- Plant Lectins
- Plants, Toxic
- Protein Conformation
- Protein Engineering
- Recombinant Fusion Proteins
- Ricin
- Ricinus
- Sequence Homology, Amino Acid