Double-site ricin B chain mutants retain galactose binding.

doi:10.1093/protein/9.4.371

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Article

Double-site ricin B chain mutants retain galactose binding.

Protein engineering - 1 Apr 1996

Frankel A, Tagge E, Chandler J, Burbage C, Willingham M

Abstract excerpt

Three distinct double-site and two single-site ricin B chain (RTB) mutants were expressed in Spodoptera frugiperda insect cells and purified from infected cell supernatants. The yields of recombinant proteins were 0.01-0.2 mg/l. The purity after monoclonal antibody affinity chromatography was 1-2...

Topics

Animals
Asialoglycoproteins
Baculoviridae
Binding, Competitive
Dose-Response Relationship, Drug
Fetuins
Galactose
Humans
Mutation
Protein Conformation
Recombinant Proteins
Ricin
Spodoptera
Toxicity Tests
Tumor Cells, Cultured
alpha-Fetoproteins

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