Article
Deletion analysis of the NH2-terminal region of beta-1,4-galactosyltransferase.
Molecular biology reports - 1 May 1992
Masibay A S, Boeggeman E, Qasba P K
Abstract excerpt
To determine the biological role, if any, of the NH2-terminal region of beta-1,4-galactosyltransferase (GT; EC 2.4.1.90), we constructed deletion mutants and expressed them in COS-7 cells. Each deletion construct was analyzed for enzymatic activity, protein production and mRNA transcription. All of the deletion mutants were transcribed to produce GT mRNA, but the GT protein was not detected in those constructs...
Topics
- Animals
- Base Sequence
- Blotting, Northern
- Blotting, Western
- Cell Line
- Cloning, Molecular
- DNA, Single-Stranded
- Genetic Vectors
- Molecular Sequence Data
- Mutation
- N-Acetyllactosamine Synthase