Article
High-level expression and simplified purification of recombinant ricin A chain.
Protein expression and purification - 1 Oct 1992
Li B Y, Frankel A E, Ramakrishnan S
Abstract excerpt
Ricin toxin is a glycoprotein which catalytically inactivates eukaryotic ribosomes by depurination of a single adenosine residue from the 28S ribosomal RNA. The enzymatic activity is present in the A chain of the toxin molecule, whereas the B chain contains two binding sites for galactose. Since...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Escherichia coli
- Genetic Vectors
- Molecular Sequence Data
- Mutation
- Plasmids
- Protein Conformation
- Recombinant Proteins
- Ricin
- Structure-Activity Relationship