Catalytic and cytotoxic activities of recombinant ricin A chain mutants with charged residues added at the carboxyl terminus.

doi:10.1006/prep.1995.1087

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Article

Catalytic and cytotoxic activities of recombinant ricin A chain mutants with charged residues added at the carboxyl terminus.

Protein expression and purification - 1 Oct 1995

Simpson J C, Roberts L M, Lord J M

Abstract excerpt

Ricin A chain (RTA) mutants which had been modified by the addition of three lysine residues, three lysines and an alanine, or six histidine residues at the carboxyl terminus were expressed in Escherichia coli. The recombinant proteins were purified to homogeneity by ion-exchange chromatography o...

Topics

Amino Acids
Animals
Cell Membrane
Chlorocebus aethiops
Chromatography
Chromatography, Affinity
Escherichia coli
Glycosides
Mutation
Plasmids
RNA, Ribosomal, 28S
Recombinant Proteins
Ricin
Triterpenes
Vero Cells

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