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Introducing site-specific glycosylation using protein engineering techniques reduces the immunogenicity of β-lactoglobulin.

Bioscience, biotechnology, and biochemistry - 1 Jan 2012

Tatsumi Yoshiyuki, Sasahara Yoshimasa, Kohyama Noriki, Ayano Satomi, Endo Michio, Yoshida Tadashi, Yamada Kiyoshi, Totsuka Mamoru, Hattori Makoto

Abstract excerpt

To reduce the immunogenicity of β-lactoglobulin (BLG), we prepared wild-type bovine BLG variant A (wt) and three site-specifically glycosylated BLGs (D28N, D137N/A139S, and P153A), and expressed them in the methylotrophic yeast Pichia pastoris by fusion of the cDNA to the sequence coding for the α-factor signal peptide from Saccharomyces cerevisiae. Sodium dodecyl sulfate polyacrylamide gel electrophoresis...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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PMID: 22451388
DOI: 10.1271/bbb.110753

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