Article
The overexpression of a Saccharomyces cerevisiae centromeric histone H3 variant mutant protein leads to a defect in kinetochore biorientation.
Genetics - 1 Feb 2007
Collins Kimberly A, Camahort Raymond, Seidel Chris, Gerton Jennifer L, Biggins Sue
Abstract excerpt
Chromosomes segregate using their kinetochores, the specialized protein structures that are assembled on centromeric DNA and mediate attachment to the mitotic spindle. Because centromeric sequences are not conserved, centromere identity is propagated by an epigenetic mechanism. All eukaryotes contain an essential histone H3 variant (CenH3) that localizes exclusively to centromeres. Because CenH3 is required for...
Topics
- Cell Cycle Proteins
- Chromosomal Proteins, Non-Histone
- DNA-Binding Proteins
- Gene Expression
- Genes, Lethal
- Histones
- Kinetochores
- Mutant Proteins
- Mutation
- Nuclear Proteins
- Phenotype
- Plasmids
- Protein Transport