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Article

Construction of mutant alleles in Saccharomyces cerevisiae without cloning: overview and the delitto perfetto method.

Current protocols in molecular biology - 11 Oct 2013

Moqtaderi Zarmik, Geisberg Joseph V

Abstract excerpt

Traditionally, methods for introducing specific new mutations at target loci in the yeast genome have involved the preparation of disruption or gene-replacement cassettes via multiple cloning steps. Sequences used for targeting these cassettes or integrating vectors are typically several hundred base pairs long. A variety of newer methods rely on the design of custom PCR oligonucleotides containing shorter...

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PMID: 24510296
DOI: 10.1002/0471142727.mb1310cs104

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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