Article
Employing libraries of zinc finger artificial transcription factors to screen for homologous recombination mutants in Arabidopsis.
The Plant journal : for cell and molecular biology - 1 Nov 2006
Lindhout Beatrice I, Pinas Johan E, Hooykaas Paul J J, van der Zaal Bert J
Abstract excerpt
A library of genes for zinc finger artificial transcription factors (ZF-ATF) was generated by fusion of DNA sequences encoding three-finger Cys(2)His(2) ZF domains to the VP16 activation domain under the control of the promoter of the ribosomal protein gene RPS5A from Arabidopsis thaliana. After introduction of this library into an Arabidopsis homologous recombination (HR) indicator line, we selected primary...
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