Article
Zinc finger artificial transcription factor-based nearest inactive analogue/nearest active analogue strategy used for the identification of plant genes controlling homologous recombination.
Plant biotechnology journal - 1 Dec 2013
Jia Qi, van Verk Marcel C, Pinas Johan E, Lindhout Beatrice I, Hooykaas Paul J J, van der Zaal Bert J
Abstract excerpt
In previous work, we selected a particular transcription factor, designated VP16-HRU, from a pool of zinc finger artificial transcription factors (ZF-ATFs) used for genome interrogation. When expressed in Arabidopsis thaliana under control of the ribosomal protein S5A promoter, the RPS5A::VP16-HRU construct led to a 200- to 300-fold increase in the frequency of somatic intrachromosomal homologous recombination...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.