Article
A library of Arabidopsis 35S-cDNA lines for identifying novel mutants.
Plant molecular biology - 1 Aug 2001
LeClere S, Bartel B
Abstract excerpt
We have developed a system to over-express or co-suppress random cDNAs in Arabidopsis thaliana upon Agrobacterium tumefaciens-mediated transformation. We constructed a binary vector containing a novel Arabidopsis cDNA library driven by the cauliflower mosaic virus (CaMV) 35S promoter. The vector, 35SpBARN, offers in terra selection with glufosinate ammonium (BASTA) and the ability to identify the cDNA insert...
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