Article
Targeted mutagenesis in Arabidopsis using zinc-finger nucleases.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011
Zhang Feng, Voytas Daniel F
Abstract excerpt
We report here an efficient method for making targeted mutations in Arabidopsis thaliana genes. The approach uses zinc-finger nucleases (ZFNs) - enzymes engineered to create DNA double-strand breaks at specific target loci. Imprecise repair of double-strand breaks by nonhomologous end-joining generates small insertions or deletions at the cleavage site. In this protocol, constructs encoding ZFNs for specific loci...
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