Article
Nuclear reprogramming of somatic cells by embryonic stem cells is affected by cell cycle stage.
Cloning and stem cells - 1 Jan 2006
Sullivan Stephen, Pells Steve, Hooper Martin, Gallagher Ed, McWhir Jim
Abstract excerpt
Hybrid embryonic stem (ES)-like clones were generated by fusion of murine ES cells with somatic cells that carried a neo resistance gene under the transcriptional control of the Oct-4 promoter. The Oct-4 promoter was reactivated in hybrid ES cells formed by fusion with fetal fibroblasts, and all hybrid colonies were of ES rather than fibroblast phenotype, suggesting efficient reprogramming of fibroblast...
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