Article
Bidirectional reprogramming of mouse embryonic stem cell/fibroblast hybrid cells is initiated at the heterokaryon stage.
Cell and tissue research - 1 Dec 2010
Gridina Maria M, Serov Oleg L
Abstract excerpt
Immunofluorescent analysis of markers specific for parental genomes was used to study heterokaryons and hybrid cells soon after the fusion of diploid embryonic stem (ES) cells marked with green fluorescent protein and diploid fibroblasts labeled by blue fluorescent beads. Heterokaryons were identified by an analysis of parental mitochondrial DNAs. Within 20 h after fusion, most heterokaryons (up to 80%) had a...
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