Article
Effect of Roscovitine‐Treated Donor Cells on Development of Porcine Cloned Embryos
10 Jul 2009
Abstract excerpt
Synchronization of the donor cell cycle is an important factor for successful animal cloning by nuclear transfer. To improve the efficiency of porcine cloning, in the present report, we evaluated effects of contact inhibition, serum starvation and roscovitine treatment of donor cells on in vitro and in vivo developmental potency of cloned porcine embryos. Fibroblasts derived from a porcine foetus at day 30 of...
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