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Article

Effects of serum starvation and re-cloning on the efficiency of nuclear transfer using bovine fetal fibroblasts

1 Mar 1999

Abstract excerpt

The developmental potential of bovine fetal fibroblasts was evaluated using nuclear transfer. Fibroblasts from a 37-day-old fetus were fused to enucleated oocytes before activation. Nuclei of starved (cultured for 8 days in medium containing 0.5% serum) fibroblasts supported the development of reconstructed embryos to the blastocyst stage significantly better than those of non-starved fibroblasts (39% versus 20%;...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 10434938
DOI: 10.1530/jrf.0.1150325

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