Mutagenic analysis of the v-crk oncogene: requirement for SH2 and SH3 domains and correlation between increased cellular phosphotyrosine and transformation.

Abstract excerpt

We have constructed a series of mutants with deletion, linker insertion, and point mutations in the v-crk oncogene of avian sarcoma virus CT10. The v-crk gene contains no apparent catalytic domain, but does contain two blocks of homology to putative regulatory domains, termed SH2 and SH3, found in a variety of proteins implicated in signal transduction. Infection with CT10 causes a dramatic increase in the level...

Topics

• Animals
• Avian Sarcoma Viruses
• Base Sequence
• Cell Adhesion
• Cell Division
• Cell Transformation, Neoplastic
• Chick Embryo
• Fibroblasts
• Molecular Sequence Data
• Mutation
• Oligonucleotide Probes