Article
Mutational activation of c-raf-1 and definition of the minimal transforming sequence.
Molecular and cellular biology - 1 Jun 1990
Heidecker G, Huleihel M, Cleveland J L, Kolch W, Beck T W, Lloyd P, Pawson T, Rapp U R
Abstract excerpt
A series of wild-type and mutant raf genes was transfected into NIH 3T3 cells and analyzed for transforming activity. Full-length wild-type c-raf did not show transforming activity. Two types of mutations resulted in oncogenic activity similar to that of v-raf: truncation of the amino-terminal ha...
Topics
- Adenosine Triphosphate
- Animals
- Binding Sites
- Blotting, Western
- Cell Transformation, Neoplastic
- Cells, Cultured
- Chromosome Deletion
- Gene Expression Regulation
- Genetic Vectors
- Mice
- Mutation
- Protein-Tyrosine Kinases
- Proto-Oncogene Proteins
- Proto-Oncogene Proteins c-raf
- Proto-Oncogenes