Article
Structural requirement of CRK SH2 region for binding to phosphotyrosine-containing proteins. Evidence from reactivity to monoclonal antibodies.
The Journal of biological chemistry - 25 Feb 1993
Matsuda M, Nagata S, Tanaka S, Nagashima K, Kurata T
Abstract excerpt
The SH2 region of signal-transducing proteins mediates binding to phosphotyrosine-containing proteins. We analyzed the structure-function relationship of the SH2 region of the human CRK protein by using a series of monoclonal antibodies (mAbs). Seventeen mAbs against the CRK SH2 region were class...
Topics
- Animals
- Antibodies, Monoclonal
- Binding Sites
- Blotting, Western
- CDC2 Protein Kinase
- Cell Line
- Chlorocebus aethiops
- Epitopes
- Humans
- Hybridomas
- Mutation
- Phosphoproteins
- Phosphotyrosine
- Protein Binding
- Proto-Oncogene Proteins
- Proto-Oncogene Proteins c-crk
- Protozoan Proteins
- Structure-Activity Relationship