Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Rapid derivation of genetically related mutants from embryonic cells harboring a recombinase-specific Trp53 platform.

Cell cycle (Georgetown, Tex.) - 15 Apr 2011

Wei Quan-Xiang, Odell Adam F, van der Hoeven Franciscus, Hollstein Monica

Abstract excerpt

Recombinase-mediated cassette exchange (RMCE) is a powerful method for achieving gene targeting repeatedly at a single mammalian locus. This approach could be applied to the efficient establishment of genetically related cell lines harboring different p53 mutations found in human tumors. To this end we generated a mouse strain called p53 Platform mice (PLF mice), containing PhiC31 integrase-specific attP...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cell Line, Transformed
Share to Electroporation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Embryonic Stem Cells

Share to Gene Targeting

Share to Integrases

Share to Microscopy, Fluorescence

Share to Protein Engineering

Share to Recombinases

Source

Identifiers

PMID: 21445009
DOI: 10.4161/cc.10.8.15303

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Rapid derivation of genetically related mutants from embryonic cells harboring a recombinase-specific Trp53 platform.PMID 21445009Read ↗

RMCE-ASAP: a gene targeting method for ES and somatic cells to accelerate phenotype analyses.Semantic proximity Read ↗

Efficient introduction of specific TP53 mutations into mouse embryonic fibroblasts and embryonic stem cells.Semantic proximity Read ↗

Insights into Wild-Type and Mutant p53 Functions Provided by Genetically Engineered MiceSemantic proximity Read ↗

Development of novel mouse models to study the p53 tumour suppressorSemantic proximity Read ↗

Generation and characterization of p53 mutant mice.Semantic proximity Read ↗

Mice carrying nonsense mutant p53 develop frequent multicentric or metastatic tumorsSemantic proximity Read ↗

Generation of a series of knock-in alleles using RMCE in ES cells.Semantic proximity Read ↗

Generation of an allelic series of knock-in mice using recombinase-mediated cassette exchange (RMCE).Semantic proximity Read ↗

Inactivation and Inducible Oncogenic Mutation of p53 in Gene Targeted PigsSemantic proximity Read ↗

Stable and efficient cassette exchange under non-selectable conditions by combined use of two site-specific recombinases.Semantic proximity Read ↗

An unstable targeted allele of the mouse Mitf gene with a high somatic and germline reversion rate.Semantic proximity Read ↗

Efficient method to generate single-copy transgenic mice by site-specific integration in embryonic stem cells.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.