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Article

Efficient introduction of specific TP53 mutations into mouse embryonic fibroblasts and embryonic stem cells.

Nature protocols - 17 May 2012

Wei Quan-Xiang, van der Hoeven Franciscus, Hollstein Monica, Odell Adam F

Abstract excerpt

This protocol describes a rapid, precise method for generating sets of embryonic stem (ES) cells or mouse embryonic fibroblasts (MEFs) harboring point mutations in the p53 tumor suppressor gene (officially known as Trp53). The strategy uses cells from the Trp53 (p53-null) 'platform' mouse, which allows site-specific integration of plasmid DNA into the Trp53 locus. Simple PCR protocols identify correctly targeted...

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Identifiers

PMID: 22596227
DOI: 10.1038/nprot.2012.042

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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