Article

Universal method for generating knockout mice in multiple genetic backgrounds using zygote electroporation

2023-04-10

Abstract excerpt

Genetically engineered mouse models are essential tools for understanding mammalian gene functions and disease pathogenesis. Genome editing allows for the generation of these models in multiple inbred strains of mice without backcrossing. Zygote electroporation dramatically removed the barrier for introducing the CRISPR-Cas9 complex in terms of cost and labour. However, the editing conditions and protocols to prod...

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Literature Corpus work: c2f2a8cf-a8b8-5ade-803e-64feae1a4179
DOI: 10.1101/2023.04.10.536207

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