Article
p53 gene targeting by homologous recombination in fish ES cells.
PloS one - 1 Jan 2013
Yan Yan, Hong Ni, Chen Tiansheng, Li Mingyou, Wang Tiansu, Guan Guijun, Qiao Yongkang, Chen Songlin, Schartl Manfred, Li Chang-Ming, Hong Yunhan
Abstract excerpt
BACKGROUND: Gene targeting (GT) provides a powerful tool for the generation of precise genetic alterations in embryonic stem (ES) cells to elucidate gene function and create animal models for human diseases. This technology has, however, been limited to mouse and rat. We have previously established ES cell lines and procedures for gene transfer and selection for homologous recombination (HR) events in the fish...
Topics
- Animals
- Blotting, Southern
- Embryonic Stem Cells
- Gene Targeting
- Genes, p53
- Genetic Engineering
- Genetic Vectors
- Genotype
- Homologous Recombination
- In Situ Hybridization
- Models, Animal
- Oryzias