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Article

Directed evolution of polymerase function by compartmentalized self-replication.

Proceedings of the National Academy of Sciences of the United States of America - 10 Apr 2001

Ghadessy F J, Ong J L, Holliger P

Abstract excerpt

We describe compartmentalized self-replication (CSR), a strategy for the directed evolution of enzymes, especially polymerases. CSR is based on a simple feedback loop consisting of a polymerase that replicates only its own encoding gene. Compartmentalization serves to isolate individual self-replication reactions from each other. In such a system, adaptive gains directly (and proportionally) translate into...

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Identifiers

PMID: 11274352
DOI: 10.1073/pnas.071052198
PMCID: PMC31872

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