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Article

Single-residue engineering of Klenow fragment enables broad acceptance of chemically modified nucleotides.

Nucleic acids research - 3 Jul 2026

Zhang Tingting, Wang Song, Xu Jiahui, Zhang Yu, Zou Haiyang, Liu Fengwu, Qin Shangshang, Herdewijn Piet

Abstract excerpt

Xeno nucleic acids (XNAs) exhibit enhanced chemical stability and significant resistance to nuclease degradation, making them attractive for synthetic biology and therapeutic development. Most engineered XNA polymerases are derived from thermophilic organisms and exhibit limited catalytic activity under physiological conditions, thereby limiting their broader application. We report a single-residue mutant (F762A)...

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PMID: 42454372
DOI: 10.1093/nar/gkag697
PMCID: PMC13369328

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