Article
Directed co-evolution of interacting protein-peptide pairs by compartmentalized two-hybrid replication (C2HR).
Nucleic acids research - 16 Dec 2020
Siau Jia Wei, Nonis Samuel, Chee Sharon, Koh Li Quan, Ferrer Fernando J, Brown Christopher J, Ghadessy Farid J
Abstract excerpt
Directed evolution methodologies benefit from read-outs quantitatively linking genotype to phenotype. We therefore devised a method that couples protein-peptide interactions to the dynamic read-out provided by an engineered DNA polymerase. Fusion of a processivity clamp protein to a thermostable nucleic acid polymerase enables polymerase activity and DNA amplification in otherwise prohibitive high-salt buffers....
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