Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Analysis of second-step mutations of class II and class III CHO aprt heterozygotes: chromosomal differences in deletion frequencies.

Somatic cell and molecular genetics - 1 May 1991

Belouchi A, Bradley W E

Abstract excerpt

We have determined the nucleotide sequence surrounding a BclI restriction fragment length variation near the aprt gene of CHO cells. By BclI digestion of the PCR-amplified DNA from a variety of APRT-deficient mutants derived from CHO cells, we were able to infer the following. First, all three he...

Topics

Adenine Phosphoribosyltransferase
Alleles
Animals
Base Sequence
Cell Line
Chromosome Deletion
Cricetinae
Cricetulus
Female
Gene Frequency
Genetic Linkage
Heterozygote

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutation

Ovary

Polymerase Chain Reaction

Polymorphism, Restriction Fragment Length

Source

Identifiers

PMID: 1675490
DOI: 10.1007/BF01232822

Genes

APRT

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1675490-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Analysis of second-step mutations of class II and class III CHO aprt heterozygotes: chromosomal differences in deletion frequencies.PMID 1675490Read ↗

Loss of alleles in aprt mutants of CHO cells demonstrated by BclI restriction-fragment-length variation.Semantic proximity Read ↗

Loss of heterozygosity in mammalian cell mutagenesis: molecular analysis of spontaneous mutations at the aprt locus in CHO cells.Semantic proximity Read ↗

Reduction to homozygosity is the predominant spontaneous mutational event in cultured human lymphoblastoid cells.Semantic proximity Read ↗

Characterization of an apparent hotspot for spontaneous mutation in exon 5 of the Chinese hamster APRT gene.Semantic proximity Read ↗

The nature of ultraviolet light-induced mutations at the heterozygous aprt locus in Chinese hamster ovary cells.Semantic proximity Read ↗

Isolation of an APRT heterozygote from TK6 human lymphoblasts: predominance of multi-locus loss of heterozygosity among spontaneous APRT-mutants.Semantic proximity Read ↗

Determination of spontaneous loss of heterozygosity mutations in Aprt heterozygous mice.Semantic proximity Read ↗

Benzo[a]pyrenediol-epoxide induces loss of heterozygosity in Chinese hamster ovary cells heterozygous at the aprt locus.Semantic proximity Read ↗

Homozygous deficiency at autosomal locus aprt in human somatic cells in vivo induced by two different mechanisms.Semantic proximity Read ↗

Genetic instability on chromosome 16 in a human B lymphoblastoid cell line.Semantic proximity Read ↗

DNA sequence analysis of mutations induced by melphalan in the CHO aprt locus.Semantic proximity Read ↗

A mutational hotspot in the aprt gene of Chinese hamster cells.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.